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A Field-Portable Bioanalysis System
2017-07-11A portable bioanalysis system provides high resolution and high detection sensitivity, and is a cost-effective solution for the field detection of pathogens. Developed by BiOptic, Inc. (New Taipei City, Taiwan), the system utilizes direct mini-PCR technology combined with a miniaturized capillary gel electrophoresis instrument in a carry-on case to target food pathogens and disease-causing viruses within about an hour, while the operator of the unit is still at the outbreak site. The system runs in real time using batteries and can interface with a computer or smartphone that analyzes the data and displays results. Applications include clinical diagnosis of viral infectious diseases in humans, plants, and animals; border security; forensics; and environmental monitoring.
NGS Library Validation Using Automated Capillary Gel Electrophoresis _ American Laboratory
2016-11-11Next-generation sequencing (NGS) is widely used in genomics, epigenetics and transcriptomics, etc.,1 and also in such clinical diagnostics applications as cancer screening and drug development.2 There are multiple commercial NGS platforms available, each incorporating unique operating principles (e.g., Roche 454FLX [454 Life Sciences, Branford, Conn.], Illumina Genome Sequencer3 [Illumina Inc., San Diego, Calif.] and Applied Biosystems/ABI SOLiD Sequencer [Foster City,Calif.]). No matter the platform, library preparation is the most critical step in the NGS workflow.
Genotyping Using CGE-A Novel Single-Channel Microfluidic Device for Nucleic Acid Fragment Analysis
2015-05-01A Novel Single-Channel Microfluidic Device for Nucleic Acid Fragment Analysis
Nucleic acid analysis, genotyping, PCR, and RFLP methodologies are time-consuming techniques using agarose or acrylamide gel electrophoresis—image capture plus gel image interpretations. Capillary gel electrophoresis (CGE) automates these, providing fast separations with high sensitivity, excellent resolution, and ease of operation. However, despite its effectiveness current CGE has a high cost of instrumentation and cost per sample. Much of this cost is due to traditional designs.
A Portable CGE-Based Biomolecule Detection System Using a Smartphone App for Operation via WiFi
2021-12-29Most bioseparation and detection tools used in the laboratory employ slab gel-based electrophoresis technologies, which have routinely been used for the analysis of biomolecules (i.e., DNA, RNA, protein, carbohydrates, etc.) since their inception more than 30 years ago. However, slab gel electrophoresis for bioanalysis is a labor-intensive technique. Further, the method needs to be drastically improved in terms of throughput, resolving power, and cost per sample run.
Disposable pen-shaped capillary gel electrophoresis cartridge for fluorescence detection
2014-03-06We introduce a novel and cost-effective capillary gel electrophoresis (CGE) system utilizing disposable pen-shaped gelcartridges for highly efficient, high speed, high throughput fluorescence detection of bio-molecules. The CGE system has been integrated with dual excitation and emission optical-fibers with micro-ball end design for fluorescence detection of bio-molecules separated and detected in a disposable pen-shaped capillary gel electrophoresis cartridge.
Use of a Pen-Shaped Capillary Gel Electrophoresis Cartridge for Cost-Effective DNA Fragment Analysis
2012-07-21Most laboratories currently utilize slab-gel electrophoresis systems for DNA and protein analysis. However, both polyacrylamide gel electrophoresis (PAGE) and agarose slab-gel electrophoresis methods are time-consuming and labor-intensive and therefore need to be improved in terms of resolving power, throughput, and cost per analysis. Capillary gel electrophoresis (CGE) has been recognized as a high-performance and automated approach that addresses the shortcomings of slab-gel electrophoresis by offering fast separations with high sensitivity, excellent resolution, ruggedness, and ease of operation.1