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CRISPR/Cas
CRISPR is a Genome Engineering technique that uses protein to interact with DNA. Genome editing begins with dsDNA cleavage. Cas9 is a dual-RNA-guided dsDNA endonuclease that is programmed by a single chimeric RNA. The guide RNA positions the Cas9 enzyme to the correct position on the target DNA for cleavage.
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cfDNA (Circulating Cell Free DNA)
Cell-free DNA (cfDNA) is known to circulate in healthy and pathological conditions and is present in plasma, serum, cerebral spinal fluid, and saliva. Nowadays, cfDNA is known to be a great target for Non-Invasive Prenatal Testing (NIPT) and Liquid Biopsy for cancer diagnosis. The quality of the cfDNA sample will strongly influence the downstream application, which makes it a very important step.
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Chromatin Immunoprecipitation (ChIP)
Chromatin immunoprecipitation (ChIP) refers to a procedure used to investigate the interaction between proteins and DNA in the cell. ChIP is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluable insights into the regulation of gene expression.
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SSR / Microsatellites
Over the years, the progress of molecular genetics methodology and the increasing availability of DNA amplification by PCR lead to widespread use of codominant molecular markers, especially microsatellites, also known as simple sequence repeats (SSRs), also known as short tandem repeats (STRs).
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Next Generation Sequencing (NGS)
Next Generation Sequencing (NGS) is a widely used technology in many fields, including genomics, epigenetics, transcriptome, etc. NGS is not only used in basic research but also applied for clinical diagnostics such as cancer screening and drug development.
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RFLP (Restriction Fragment Length Polymorphism)
Restriction fragment length polymorphism (RFLP) is considered to be the simplest and earliest method to detect SNPs. SNP-RFLP makes use of the many different restriction endonucleases and their high affinity to unique and specific restriction sites.
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Immuno Assay
Immunoassays are commonly used in biotechnology for the detection and quantification of host cell contaminants. The free-solution approach by CGE with fluorescence detection offers an exciting alternative to solid-phase immunoassay. It eliminates antigen immobilization and avoids many problems associated with solid phase. The methodology makes use of either a purified antigen labeled with stable fluorescent dye (i.e., FITC) or an affinity probe labeled with the dye (direct assay).
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Glycan
BiOptic Inc. introduces a cost-effective capillary gel-electrophoresis system for highly efficient, high speed, high throughput, Glycan analysis (N-Glycan). The Glycan Analyzer (GL1000) is a newly developed product that will significantly increase the pace at which glycoprotein research is performed in the lab, saving hours of preparation time and assuring accurate, consistent and economical results.