On-Site Testing of Infectious Diseases: SARS-CoV-2
Background: Nucleic acid detection by RT-PCR method is one of the criteria approved by China FDA and US CDC for diagnosis of COVID-19, which requires highly trained personnel and can create inaccurate test results (for example, high false-negative rate and some false positive rate). Inaccurate results are caused by inadequate detection sensitivity of RT-PCR, low viral load in some patients, difficulty to collect samples from COVID-19 patients, insufficient sample loading during RT-PCR tests, and RNA degradation during the sample handling process.
