Restriction fragment length polymorphism (RFLP) is considered to be the simplest and earliest method to detect SNPs. SNP-RFLP makes use of the many different restriction endonucleases and their high affinity to unique and specific restriction sites.
On-Site Testing of Infectious Diseases: SARS-CoV-2
Background: Nucleic acid detection by RT-PCR method is one of the criteria approved by China FDA and US CDC for diagnosis of COVID-19, which requires highly trained personnel and can create inaccurate test results (for example, high false-negative rate and some false positive rate). Inaccurate results are caused by inadequate detection sensitivity of RT-PCR, low viral load in some patients, difficulty to collect samples from COVID-19 patients, insufficient sample loading during RT-PCR tests, and RNA degradation during the sample handling process.
Degradation of RNA samples by RNase is a major cause of failure in the following experiments. The Qsep series bio-fragment analyzers can easily detect even slight degradation and display the results of RNA quality by gel-like profiling and Electropherogram.
SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species. Single-nucleotide polymorphisms (SNPs) are widespread genomic variations, which are associated with serious health disorders and drug resistance.
