Direct SARS-CoV-2 Detection System Utilizing Simple-to-Use Capillary Gel Electrophoresis

Direct SARS-CoV-2 Detection System Utilizing Simple-to-Use Capillary Gel Electrophoresis Sample-to-Result
Author(s): Neo Yang, Meya Kuo, Jerry Liu, Varoujan Amirkhanian* and Eric Tsai
DOI: 10.2174/1566524023666221104160148

We present a Direct SARS-CoV-2 Detection System that achieves sample-to-results in less than two hours in three simple steps. The Detection System includes Direct one-step Reverse Transcription PCR (RT-PCR) reagents (Qexp-MDx kit), a portable thermal cycler (Qamp-mini) with a pre-programmed chip, and a simple-to-use Capillary Gel Electrophoresis system (Qsep Series Bio-Fragment Analyzer) with high fluorescence detection sensitivity to solve the problems associated with traditional real-time PCR (qPCR) systems which produce inaccurate test results with high false negative and false positive rates. The proposed simple-to-use detection platform can provide high detection sensitivity (identify less than 20 copies), fast results (less than 120 minutes), and cost-effective results which should be suitable for decentralized testing applications of COVID-19. 

Keywords: Polymerase Chain Reaction (PCR), Reverse Transcription PCR (RT-PCR), Gel Electrophoresis, Capillary Electrophoresis (CE), Fragment Analyzer, Pandemic, SARS-CoV-2, Coronavirus, COVID-19

Reference: https://www.eurekaselect.com/article/127412

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