Bioinformatic analysis of peripheral blood miRNA of breast cancer patients in relation with
2020-03-03
The current diagnostic methods and treatments still fail to lower the incidence of anthracycline-induced cardiotoxicity effectively. In this study, we aimed to (1) analyze the cardiotoxicity-related genes after breast cancer chemotherapy in gene expression database and (2) carry out bioinformatic analysis to identify cardiotoxicity-related abnormal expressions, the biomarkers of such abnormal expressions, and the key regulatory pathways after breast cancer chemotherapy.
Evaluation of DNA degradation and establishment of a degradation analysis model for
2020-01-28
Millions of museum specimens are integral to biodiversity studies; however, DNA degradation may limit the ability to obtain DNA sequences. In this study, a degradation analysis model for Lepidoptera specimens was established. Based on this model, we revealed the characteristics of DNA
Targeted sequencing and RNA assay reveal a noncanonical JAG1 splicing variant
2020-01-24
Alagille syndrome (ALGS), as known as congenital arteriohepatic dysplasia, is a rare autosomal dominant multi-systemic disorder. Mutations in JAG1 or more rarely NOTCH2 have been reported as the cause of ALGS. In this study, a 5-year old girl with typical ALGS feature and her pregnant mother came to our reproductive genetics clinic for counseling. We aimed to clarify the genetic diagnosis and provide prenatal genetic diagnosis for the pregnant. Next generation sequencing (NGS)-based multigene panel was used to identify pathogenic
Genetic diversity of Trachycarpus fortunei（Hook.）H. Wendl germplasm resources in Guizhou by SRAP
2020-01-04
The study on genetic diversity and genetic structure of Trachycarpus fortunei（Hook.）H. Wendl resources in Guizhou，revealed its distribution pattern and variation trend，and provided scientific basis for the protection， development and utilization of T. fortunei germplasm resources in Guizhou. [Method] Based on SRAP molecular markers， 328 materials from 26 T. fortunei provenances in 7 agroclimatic regions of Guizhou were amplified. After electrophoresis analysis by Qsep 100 automatic nucleic acid protein analyzer，Popgene 1.32 and NTSY
