Applications

Genomic DNA (gDNA) Analysis

Genomic deoxyribonucleic acid is chromosomal DNA. Is also abbreviated as gDNA. Most organisms have the same genomic DNA in every cell; however, only certain genes are active in each cell to allow for cell function and differentiation within the body.


The accurate assessment of genomic DNA size, quantity, and quality is crucial to downstream applications including: PCR, genotyping, and next-generation sequencing (NGS). Genomic DNA whether intact, FFPE or from ancient samples provides scientists with insight into the underlying biology of cancer and other diseases within populations, and so much more.


The genome of an organism (encoded by the genomic DNA) is the (biological) information of heredity which is passed from one generation of organism to the next. That genome is transcribed to produce various RNAs, which are necessary for the function of the organism. Precursor mRNA (pre-mRNA) is transcribed by RNA polymerase II in the nucleus. pre-mRNA is then processed by splicing to remove introns, leaving the exons in the mature messenger RNA (mRNA). Additional processing includes the addition of a 5' cap and a poly(A) tail to the pre-mRNA. The mature mRNA may then be transported to the cytosol and translated by the ribosome into a protein. Other types of RNA include ribosomal RNA (rRNA) and transfer RNA (tRNA). These types are transcribed by RNA polymerase II and RNA polymerase III respectively and are essential for protein synthesis. However 5s rRNA is the only rRNA which is trancribed by RNA Polymerase III.


Qsep1, Qsep100 or Qsep400 Bio-Fragment Analyzers are simple-to-use CGE instruments that could be utilized for the QC testing of gDNA size and concentration of fragments.

 

Test Setup:

• Cartridge: S2-O-170612-1 
• Alignment marker: 20-5Kb 
• Method: M4-10-08-200 
• Average Size: 857 bp