Immunoassays are commonly used in biotechnology for the detection and quantification of host cell contaminants. The free-solution approach by CGE with fluorescence detection offers an exciting alternative to solid-phase immunoassay. It eliminates antigen immobilization and avoids many problems associated with solid phase. The methodology makes use of either a purified antigen labeled with stable fluorescent dye (i.e., FITC) or an affinity probe labeled with the dye (direct assay).

Immuno Assay Test: Antigen and Antibody Reaction

Figure 1: Bioptic's CGE analysis result for immuno-assay


Figure 2: BiOptic's CGE analysis result of reducing antibody