Plasmid is commonly found as small, circular, independent, self-replicated double stranded DNA separated from chromosome DNA in bacteria. Its size ranges from 1- 100Kbp and is known to carry genes that confer antibiotic resistance which are used in genetic therapy to cure many diseases. Plasmids can be rapidly exchanged between bacterium through pilus and spread the gene into new hosts during conjugation. It can be transmitted from one bacterium to another even in different species: archaea and eukaryotic organisms. Its famous known function is carrying antibiotic resistance gene to cure many diseases.
Plasmids are widely used in fields of genetic research like gene therapy and recombinant DNA technologies for many purposes depending on the type of plasmids. There are five types of Plasmids: Resistance ®-plasmids, c, fertility plasmids, col plasmids and virulence plasmids.
BiOptic's Qsep100-- provides programs that can be custom designed for different experimental purposes. For example, Figure 1 illustrate the plasmid restriction map time course experiment and visualize the restriction mechanism of the plasmid digestion time course. This helps researchers to see how the digestion enzymes affect plasmids at different times. This program's design was based on a particular client's need. You can always add additional time ranges and decide how many sampling time points you need. Therefore, all the experiments are done automatically in the same environment to reduce the presence of nuisance factors that affect data quality.
With the unique function of the Qsep100, it makes the time-course test easier than ever.
Figure1: The pBR322 plasmid DNA was digested by Msp1 enzyme in room temperature to show the mechanism of the cutting process. The pBR322-MspI digestion process indicates that plasmid was initially cleaved into linear form of a certain length (about ≤ 4000bp) and finally chopped into small pieces of DNA fragments. The first analysis result at 0min is original sample starts being digested. It shows pBR322 Plasmid linear and OC peaks.