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Oligonucleotides are referred to short nucleic acid polymers and usually made up with twenty or fewer bases. It can be formed by bond cleavage of longer segments. However, nowadays it's more commonly synthesized up to 160 to 200 bases by automated synthesizers. The length of the oligonucleotide is denoted by "mer". For instance, a fragments of 17 bases is called a 17-mer.  Oligonuleotide is wide applied as role called probes for detecting DNA or RNA due to its ability to bind to complementary nucleotides. Therefore, it's commonly used in many scientific techniques such as PCR , DNA microarrays, southern blots, ASP analysis, fluorescent in situ hybridization (FISH), and synthesis of artificial genes. 

 

Using Qsep100 to conduct the QC test for Oligonucleotides.

    1

ACAGC ATCGC CAGTC ACTAT

21 mer

   2

CCTGT GGCGC CGGTG ATG  

18 mer

    3 TGGCC TGCTT CTCGC CG  17mer


Figure1: Qsep100 CGE fragment analyzer precisely separated the three primers as 17mer, 18 mer and 21 mer.